Q&A

A method to help detect genotoxic impurities (GTIs) using headspace gas chromatography (HS-GC) and deep eutectic solvents (DESs) has been developed.

Peter Pellegrinelli, Applications Specialist at Advanced Materials Technology (AMT) explains the complexity of oligonucleotide separations due to the unique chemical properties of these molecules. Issues such as varying length, sequence complexity, and hydrophilic-hydrophobic characteristics make efficient separations difficult. Separation scientists are addressing these challenges by modifying mobile phase compositions, using varying ion-pairing reagents, and exploring alternative separation modes like HILIC and ion-exchange chromatography. Due to these complexities, AMT has introduced the HALO® OLIGO column, which offers high-resolution, fast separations through its innovative Fused-Core® technology and high pH stability. Alongside explaining the new column, Peter looks to the future of these separations and what is next to come.

This app note describes a non ion-pairing reversed-phase LC/MS method for highly modified ASOs, DNA, sgRNA, and siRNA ranging from 14- to 103-mer oligonucleotides.

A European Union reference laboratory recently created a new method for analyzing gas chromatography (GC)-amenable pesticides in foods of animal origin.

Spanish scientists recently tested flow injection analysis–mass spectrometry (FIA–MS) and its effectiveness in detecting fraudulent substances in Coleus forskohlii food supplements.