Pegasus 4D GCxGC-TOFMS
LECO product profile
Variable Modulation Times (VMT) for Pegasus® 4D GCxGC-TOFMS optimisation is a unique feature designed and implemented by LECO. Traditional GCxGC uses a fixed modulation period and can result in an analytical compromise to the 1st dimension separation. The advantage of VMT enables the operator to specify at the click of a button their own and different modulation times throughout the GCxGC run time. Typically, applications taking advantage of GCxGC separations focus on matrix heavy &/or complex samples containing a broad range of analytes. In such complex mixtures, early eluting and more volatile analytes actually require shorter modulation times whilst later eluting analytes (high boilers) require much longer modulation times. Using the new LECO VMT programming style results in graduated Pegasus 4D® GCxGC-TOFMS chromatograms in the 2nd dimension and so preserves the valuable separation in the first dimension. This feature maximizes resolution in both the first and second dimensions and is compatible with LECO’s traditional LN2 and Consumable Free (CF) Pegasus 4D® GCxGC-TOFMS instruments.
The newly implemented features are built on a history of dedication and continuous development ensuring LECO continues to provide the gold standard of GCxGC instrumentation, the Pegasus 4D® GCxGC-TOFMS. For more information contact the LECO Separation Science team.
LECO European Technical Centre (ETC)
Marie-Bernays-Ring 31
41199 Mönchengladbach
Germany
Tel: +49 (0)2166 687 -104 or -107
Email: SepSci@leco.de
Web: www.leco.com
The Complexity of Oligonucleotide Separations
January 9th 2025Peter Pellegrinelli, Applications Specialist at Advanced Materials Technology (AMT) explains the complexity of oligonucleotide separations due to the unique chemical properties of these molecules. Issues such as varying length, sequence complexity, and hydrophilic-hydrophobic characteristics make efficient separations difficult. Separation scientists are addressing these challenges by modifying mobile phase compositions, using varying ion-pairing reagents, and exploring alternative separation modes like HILIC and ion-exchange chromatography. Due to these complexities, AMT has introduced the HALO® OLIGO column, which offers high-resolution, fast separations through its innovative Fused-Core® technology and high pH stability. Alongside explaining the new column, Peter looks to the future of these separations and what is next to come.
Metabolomics Analysis of Low Birth-Weight Infants Using UHPLC-MS/MS Following Lipid Emulsion
January 10th 2025A recent study aimed to directly compare the changes in serum metabolites among very low birth-rate (VLBW) infants following the administration of the soybean oil-based lipid emulsion and soybean oil, medium-chain triglycerides, olive oil, and fish oil (SMOF) lipid emulsion using untargeted metabolomics techniques.
Analyzing New Drug Modalities: An ISC 2024 Interview with Kelly Zhang
January 10th 2025At ISC 2024 in Liverpool, United Kingdom, LCGC International interviewed Kelly Zhang of Genentech about her work analyzing new drug modalities, such as mRNA, oligonucleotides, peptides, and cell and gene therapies.
