New Stationary Phases for GC and LC
Pittcon 2009 has brought us back to the windy city, where the technical sessions are sure to be anything but breezy. Sunday afternoon's session titled, "New Stationary Phases for GC and LC," is a prime example of a session that was filled with new information for separation scientists to enjoy.
Pittcon 2009 has brought us back to the windy city, where the technical sessions are sure to be anything but breezy. Sunday afternoon’s session titled, “New Stationary Phases for GC and LC,” is a prime example of a session that was filled with new information for separation scientists to enjoy.
The session began with “Hybrid Mid Polarity Siloxane Stationary Phase Polymers for Next Generation High Performance Capillary GC Columns,” presented by Jack Cochran of Restek Corporation, along with Gary Stidsen, Roy Lautamo, Shawn Reese, Jaap de Zeeuw, Jason Thomas, and Jari Snider.
Following that 20-minute presentation was Allen J. Britten from Cape Breton University and Krishnat P. Naikwadi presenting their findings on “Isomer Specific and High Temperature New Nano Stationary Phase GC Capillary Columns for Fast GC and GC/MS Analysis.”
Third in this session was a presentation titled “LC/MS of Metabolites using a Silica Hydride-Based Stationary Phase.” Joseph Pesek, of San Jose State University, and Maria Matyska presented.
Rounding out this session was Abby Jo Jackson, from the University of Nebraska-Lincoln, along with Hai Xuan, and David S. Hage presenting their findings on “Entrapment of Proteins in Polysaccharide-Capped Hydrazie-Activated Silica Supports for High Performance Affinity.” All-in-all it was a very informative session to start out was is sure to be a great week in Chicago.
Inside the Laboratory: The Zydney Group at Pennsylvania State University
November 26th 2024In this edition of “Inside the Laboratory,” Andrew Zydney of Pennsylvania State University discusses his laboratory’s work with using high performance liquid chromatography (HPLC) to analyze biopharmaceutical products before and after membrane separation processes.
AI and GenAI Applications to Help Optimize Purification and Yield of Antibodies From Plasma
October 31st 2024Deriving antibodies from plasma products involves several steps, typically starting from the collection of plasma and ending with the purification of the desired antibodies. These are: plasma collection; plasma pooling; fractionation; antibody purification; concentration and formulation; quality control; and packaging and storage. This process results in a purified antibody product that can be used for therapeutic purposes, diagnostic tests, or research. Each step is critical to ensure the safety, efficacy, and quality of the final product. Applications of AI/GenAI in many of these steps can significantly help in the optimization of purification and yield of the desired antibodies. Some specific use-cases are: selecting and optimizing plasma units for optimized plasma pooling; GenAI solution for enterprise search on internal knowledge portal; analysing and optimizing production batch profitability, inventory, yields; monitoring production batch key performance indicators for outlier identification; monitoring production equipment to predict maintenance events; and reducing quality control laboratory testing turnaround time.
Merck: Obtaining Ultrapure Water for Sensitive PFAS Analysis by LC-MS
November 26th 2024Ultrapure water from a Milli-Q® water purification system was tested for PFAS according to the draft EPA 1633 method. None of the 40 PFAS compounds tested were detected in the ultrapure water produced by a Milli-Q® IQ 7000 system fitted with a LC-Pak® polisher at the point-of-dispense, making it suitable for the most sensitive PFAS analyses by LC- MS/MS.
Measuring Stress and Reproductive Hormones in Dolphins with UHPLC
November 25th 2024A recent study measured stress and reproductive hormones in three stocks of free-ranging bottlenose dolphins inhabiting different natural salinities across the Gulf of Mexico, with hormones extracted from the blubber of 82 remotely biopsied dolphins and quantified using ultrahigh-pressure liquid chromatography (UHPLC) coupled with orbital ion trap fusion mass spectrometry.
