New Saccharide Determination HPLC-UV System

A team of scientists from Nanjing, China, have created a low-cost efficient online derivatization system, which they used to detect mono- and oligo-saccharides using a high-performance liquid chromatography (HPLC)-ultraviolet (UV) detector system. Their work was recently published in the Journal of Separation Science (1).

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This method uses phenylhydrazine as the derivatization reagent, meaning it was directly spiked in the mobile phase to allow for the separation and detection of mono- and oligosaccharides in the HPLC-UV system. Further, the method’s online derivatization design significantly reduced the likelihood of analysts being harmed of derivatization reagents (1). Chromatographic parameters were optimized using the Box-Behnken design strategy, which is a quadratic design that lacks an embedded factorial design (2).

After conducting different experiments, the proposed method showed satisfactory results. The linear ranges held acceptable correlation coefficients (R2 > 0.99), accuracy (Recovery: 95.3%–105.6%), high intra-day precision (relative standard deviation [RSD]: 1.4%–7.1%) and inter-day precision (RSD: 2.0%–7.4%). Meanwhile, the recovery values were in the ranges of 95.0%–104.6% and 95.1%–104.8% for robustness and ruggedness, respectively. These results encouraged the scientists’ belief in the applicability and reliability of the proposed method, showing potential for analyzing saccharides in complex real-world samples.

References

(1) Jiang, Z.; Wang, H.; Yu, M.; Qu, C.; Yue, W.; Wu, Q. A low-cost efficient online derivatization system for the determination of saccharides by high-performance liquid chromatograph-ultraviolet detector. J. Sep. Sci. 2023, 46 (20), 2300384. DOI: https://doi.org/10.1002/jssc.202300384

(2) Box-Behnken Designs. NIST 2023. https://www.itl.nist.gov/div898/handbook/pri/section3/pri3362.htm#(accessed 2023-10-30)