Modern HPLC Strategies: Improving Retention and Peak Shape for Basic Analytes

Sartorius recently announced the release of its new Vivaflow® Tangential Flow Filtration (TFF) Cassettes, which aim to streamline tangential flow filtration (TFF) in the laboratory.

This year, the ISC’s theme is “Imagine a world of Chromatography” will see conference attendees discuss a wide variety of disciplines where chromatography plays a crucial role.

Petroleum contamination from leaking underground storage tanks, for example, is a significant concern for both the environment and human health. Thorough characterization of the contamination is required to form appropriate risk assessments and remediation strategies, but until now, the determination of total petroleum hydrocarbons (TPHs) in soil has typically involved a convoluted and labour-intensive process. In this article, the analysis of TPH in environmental media is simplified using flow-modulated GC×GC–FID with quantitation based on pre-defined compound groupings. This approach overcomes the drawbacks of conventional solvent fractionation approaches, by eliminating the need for sample fractionation and automating data processing workflows.

Column insertion distance is critical to good chromatography. What happens if the column is installed too low in the injection port? Is insertion distance more important when performing split injection or splitless injection? Does the position of the column in the injection port impact reproducibility?