Measuring Physical Properties of Liposomes and LNPs for RNA Delivery with Multi-Detector Asymmetric-Flow Field-Flow Fractionation

Register free: https://www.chromatographyonline.com/lcgc_w/fractionation

Event Overview:
Multi-detector asymmetric-flow field-flow fractionation (MD-AF4) has been recognized as an invaluable tool for the characterization of particle size, polydispersity, drug loading and stability of nanopharmaceuticals. The application of robust and high-quality standard operating procedures (SOPs) is critical for accurate and reliable measurements, especially as these complex drug nano-formulations are most often inherently polydisperse.

A unique international collaboration, involving two state-of-the-art infrastructures in the field of nanomedicine—the European Nanomedicine Characterization Laboratory (EUNCL) and the National Cancer Institute Nanotechnology Characterisation Laboratory (NCI-NCL)—has led to the development of a robust SOP for the measurement of physical-chemical properties of nanopharmaceuticals by MD-AF4. After a brief overview of MD-AF4 principles and instrumentation, this webinar will present examples of how MD-AF4 quantifies key quality attributes of complex nanomedicine formulations such as particle size distributions, shape, concentration and stability, using the SOP guidelines. Particular focus will be given to the characterisation of liposomal products and of lipid-based nanoparticles (LNPs) for RNA delivery. Finally, current efforts within ASTM for standardisation of methods based on MD-AF4 will be presented.

Key Learning Objectives:

• Method optimization of MD-AF4 for liposomes and LNPs for RNA delivery
• Method validation according to ISO TS 21362:2018
• Measurement of particle sizing for batch-to-batch variability assessment and particle stability in complex media
• Measurement of particle concentration

Speakers: Fanny Caputo, Ph.D., Research Scientist, Dept. of Biotechnology and Nanomedicine
SINTEF Industry

Christoph Johann, Global Product Manager, Wyatt Technology

Time and Date: Asia Pacific: Thursday, May 27, 2021 at 9am CST | 10am JST | 11am AEST

Register free: https://www.chromatographyonline.com/lcgc_w/fractionation