LC–MS, Bioanalytical

Session 410, Room 209A.

This Monday morning session will be presided over by Elizabeth Harris (Mankind Corporation).

The session will begin with a presentation by Edward Franklin of the University of North Carolina at Chapel Hill (Chapel Hill, North Carolina) titled “Long Microcapillary Columns at Elevated Temperatures and Pressures for Use in Proteomic and Lipidomic Applications. Joining Franklin will be James W. Jorgenson, winner of the 2011 LCGC North America Lifetime Achievement Award. Franklin’s discussion describes efforts to fabricate and characterize long microcapillary columns packed with 1.7-µm particles.

The next presentation in the session is titled “Characterization of Post-Source Fragmentation with Deconvolution and Accurate Mass Measurement as a Tool for Analyte Identification in Natural Products: HPLC–TOF–MS Analysis with Database Searching of Fragment Ions” and will be delivered by Jeffrey S. Patrick of LECO Corporation (St. Joseph, Michigan). Patrick will discuss how the growing presence of hybrid instruments, which provide both MS-MS and accurate mass information, has created more opportunities for analyte identification.

Eduard Rogatsky, of Albert Einstein College of Medicine (Bronx, New York) will continue the session with “3D LC–MS for Better Sensitivity, Reproducibility, and Ruggedness.” Rogatsky will discuss the popularity of high-speed HPLC based in fused-core particle design in LC–MS analysis, its limitations, and the benefits of column switching to overcome small sample loading capacity in the analysis of complex biological samples.

Holly M. Shackman, of Bristol-Myers Squibb (New York, New York) follows with a presentation titled “Recognizing Quaternary Amines Using Electrospray Mass Spectrometry.” Shackman’s discussion will look at studies performed to develop a method for rapidly and unambiguously identifying quaternary amines for structural analysis.

Following a 15-minute recess, Buu Tran, of the Wadsworth Center (Albany, New York) will present “Trace Analysis of Zearalenone and Its Analogs in Food Matrices by Solid Phase Extraction-Liquid Chromatography Tandem Mass Spectrometry,” providing insight into the results of a study that extracted ultratrace amounts of zearalenone and its analogs including α- and β-zearalenone, α- and β-zearalanone in food matrices using solid-phase extraction by liquid chromatography–electrospray tandem mass spectrometry.

Justin C. Cooley, of the University of Kansas (Lawrence, Kansas) is up next with a presentation titled “Development of an LC-MS/MS Method for the Detection of Arachidonic Acid Metabolites in Microdialysis Samples.” He will describe an HPLC–MS-MS method for the detection of metabolites along the AA cascade, specifically, 6-ketoPGF1 alpha, TXB2, 8-isoPGF2α, PGF2α, PGE, PGD, LTB4, and 12-HETE.

Barbara Bojko, of the University of Waterloo (Waterloo, Ontario, Canada), will present next, with “Therapeutic Monitoring of Tranexamic Acid Concentration in Patients Undergoing Cardiac Surgery with the Use of Cardiopulmonary Bypass.” She will report about a pharmacokinetic profile obtained from the prospective study performed on a group of patients who underwent heart surgery with the use of cardiopulmonary bypass, which was compared with the theoretical model currently used as an established dosing regime of tranexamic acid in cardiac surgery.

The final presenter of this session will be Miriam Schwarzer, of the University of Muenster (Muenster, Germany), whose talk is titled “Investigation of the Adduct Formation of Mercury Species with Components of Cell Culture Media by Means of HPLC/ESI-TOF-MS.” The talk explores the adduct formation of ethyl- and methylmercury and inorganic mercury with fetal calf serum and small thiols by means of HPLC coupled to electrospray time-of-flight mass spectrometry as well as inductively coupled plasma mass spectrometry.