LC-MS Used in Simultaneous Quantification of Three Iridoids in Rat Plasma
Scientists Zhi-Min Long, Kai-Shun Bi, Yan-Shuang Huo, Xiao-Yu Yan, Xu Zhao, and Xiao-Hui Chen at Shenyang Pharmaceutical University, (Shenyang, China) developed and validated a sensitive and efficient liquid chromatography-mass spectrometry (LC-MS) method for the simultaneous determination of geniposide 6a-hydroxygeniposide, and genipin gentiobioside in rat plasma.
Scientists Zhi-Min Long, Kai-Shun Bi, Yan-Shuang Huo, Xiao-Yu Yan, Xu Zhao, and Xiao-Hui Chen at Shenyang Pharmaceutical University, (Shenyang, China) developed and validated a sensitive and efficient liquid chromatography-mass spectrometry (LC-MS) method for the simultaneous determination of geniposide 6α-hydroxygeniposide, and genipin gentiobioside in rat plasma.
After the addition of internal standard salidroside and acidification (formic acid, 0.1%), plasma samples were carried out by protein precipitation with acetonitrile and separated on a C18 column (200 mm×4.6 mm, 5 μm) within a runtime of 15.0 min. The linear ranges were 2–250 ng/mL for both 6α-hydroxygeniposide and genipin gentiobioside and 2–2000 ng/mL for geniposide, respectively.
The validated method was successfully applied to a pharmacokinetics study of geniposide, 6α-hydroxygeniposide, and genipin gentiobioside in rats after oral administration of a Zhi-zi-chi (gardenia and prepared soybean) decoction.
The abstract was published in the October 2011 issue of Journal of Separation Science, Volume 34, pp 2854-2860.
Fundamentals of Benchtop GC–MS Data Analysis and Terminology
April 5th 2025In this installment, we will review the fundamental terminology and data analysis principles in benchtop GC–MS. We will compare the three modes of analysis—full scan, extracted ion chromatograms, and selected ion monitoring—and see how each is used for quantitative and quantitative analysis.
Characterizing Plant Polysaccharides Using Size-Exclusion Chromatography
April 4th 2025With green chemistry becoming more standardized, Leena Pitkänen of Aalto University analyzed how useful size-exclusion chromatography (SEC) and asymmetric flow field-flow fractionation (AF4) could be in characterizing plant polysaccharides.
This information is supplementary to the article “Accelerating Monoclonal Antibody Quality Control: The Role of LC–MS in Upstream Bioprocessing”, which was published in the May 2025 issue of Current Trends in Mass Spectrometry.
