LC Columns for Biomolecule Separations
Analytical and nano scale Thermo Scientificâ„¢ Accucoreâ„¢ 150-C18 and 150-C4 columns are rugged and easy to use. The columns are designed to improve protein and peptide assay robustness by enhancing peak capacity and run times at moderate operating pressures.
Analytical and nano scale Thermo Scientific™ Accucore™ 150-C18 and 150-C4 columns are rugged and easy to use. The columns are designed to improve protein and peptide assay robustness by enhancing peak capacity and run times at moderate operating pressures.
The 150 angstrom pore diameter of the solid core particles are designed to provide the optimal combination of retention time and resolution for peptides and proteins. The Accucore 150-C18 has been further optimized for biomolecules and protein digests through bonding of C18 ligands onto the porous outer layer of the particles. The low hydrophobicity C4 ligand used in the Accucore 150-C4 column enables fast protein separations.
Advanced bonding technology produces excellent peak shapes through high bonding coverage and minimized secondary interactions. The automated packing processes produce uniform, tightly-packed columns which are robust and reproducible.
ref=http://info1.thermoscientific.com/forms/CMD_Accucore150_E861?ce=E.12CMD.CC119.G038650.00&gp=MOA-Media%20Online%20Adv> Learn more about Accucore HPLC columns for biomolecule separations
The Complexity of Oligonucleotide Separations
January 9th 2025Peter Pellegrinelli, Applications Specialist at Advanced Materials Technology (AMT) explains the complexity of oligonucleotide separations due to the unique chemical properties of these molecules. Issues such as varying length, sequence complexity, and hydrophilic-hydrophobic characteristics make efficient separations difficult. Separation scientists are addressing these challenges by modifying mobile phase compositions, using varying ion-pairing reagents, and exploring alternative separation modes like HILIC and ion-exchange chromatography. Due to these complexities, AMT has introduced the HALO® OLIGO column, which offers high-resolution, fast separations through its innovative Fused-Core® technology and high pH stability. Alongside explaining the new column, Peter looks to the future of these separations and what is next to come.
Metabolomics Analysis of Low Birth-Weight Infants Using UHPLC-MS/MS Following Lipid Emulsion
January 10th 2025A recent study aimed to directly compare the changes in serum metabolites among very low birth-rate (VLBW) infants following the administration of the soybean oil-based lipid emulsion and soybean oil, medium-chain triglycerides, olive oil, and fish oil (SMOF) lipid emulsion using untargeted metabolomics techniques.
Analyzing New Drug Modalities: An ISC 2024 Interview with Kelly Zhang
January 10th 2025At ISC 2024 in Liverpool, United Kingdom, LCGC International interviewed Kelly Zhang of Genentech about her work analyzing new drug modalities, such as mRNA, oligonucleotides, peptides, and cell and gene therapies.
