HPLC Troubleshooting Guide to Cycling Baselines and Pressure Fluctuations
HPLC troubleshooting guide to cycling baselines and pressure fluctuations from LCGC's ChromAcademy.
The Problem
A short term cycling baseline is seen along with pressure fluctuations (Figure 1).
Figure 1: Shor-term cycling baseline.
The CHROMacademy HPLC Troubleshooter
Both chromatographic (up to three) and instrument symptoms (up to two) can be selected at one time. The chromatographic symptom which is being seen is a short term cycling baseline along with pressure fluctuations, which is instrument related (Figure 2). Each option has a helpful rollover which illustrates the problem; this can be useful if you are not entirely sure what problem you are seeing.
Figure 2: Input of chromatographic (top) and instrument (bottom) symptoms into the CHROMacademy HPLC Troubleshooter.
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Reversed Phase HPLC for the Analysis of Biomolecules
November 15th 2016Biopharmaceuticals offer great hope in treating medical conditions which are currently poorly served, at best, by traditional pharmaceuticals. It is estimated that there are over 400 biopharmaceuticals in clinical trials for in excess of 200 disease areas. The enhanced complexity and variability that comes from the size of biopharmaceuticals, allied with the intricacy of the production process, mean chromatography is employed to a much greater extent during production and release testing. The following article will introduce the fundamentals of biopharmaceutical analysis and cover the use of reversed phase HPLC in the analysis of biomolecules. A subsequent article will detail the application of HILIC, IEX, and SEC chromatography for the analysis if biomolecules.
