HPLC Analysis of Oligonucleotides

This webinar will provide an overview of the HPLC analysis of oligonucleotides and the basics of how synthetic oligonucleotides are produced. Additionally, both anion exchange and ion-paired reversed phase analysis will be examined, moving on to an introduction to the application of mass spectrometry to oligonucleotides.

Register Free:
https://www.chromatographyonline.com/lcgc_w/oligonucleotides-analysis

Event Overview:

This session will provide an overview of the high-performance liquid chromatography (HPLC) analysis of oligonucleotides. The presentation will review the basics of nucleotides and how synthetic oligonucleotides are produced, introducing the audience to some of the language used in support of these molecules. Additionally, both anion exchange and ion-paired reversed phase analysis will be examined, moving on to an introduction to the application of mass spectrometry (MS) to oligonucleotides.

Topics Covered:

• Biotechnology
• Oligonucleotides overview
• Therapeutic oligonucleotides
• Oligonucleotide synthesis
• Oligonucleotide chromatographic considerations
• Anion exchange chromatography
• Ion pair reversed phase chromatography
• Mass spectrometry considerations
• Ion pair-MS
• Intact mass spectrum
• IP-MS/MS

Speaker:

Colin Towers
Senior Technical Support Chemist
Element

After completing a degree in pharmacy, Colin pursued a career in analytical chemistry. Following three years performing routine testing and three years instrument maintenance and calibration, he moved into a role as a method development and validation specialist in contract research. Gaining 11 years of experience in developing methods for HPLC, LC-MS, GC, GC-MS, and SPE in a high-throughput commercial environment, this was supplemented by three years lecturing in forensic analytical toxicology, as well as a further three years working as a community pharmacist. Colin has been with Element since 2011 as a technical specialist, consultant, and trainer.

Register Free: https://www.chromatographyonline.com/lcgc_w/oligonucleotides-analysis