HPLC 2015 Wednesday Morning Sessions
HPLC2015 Wednesday Morning Sessions
Session 25: Fundamentals
Room 1, Level 1
08:30 -Mark Schure (Uwe D. Neue Award Recipient): Insights into Chromatographic Processes through Particle-Based Simulation Methods
09:00 -Szabolcs Fekete: Estimation of Frictional Heating and Temperature Related Effects on the Retention of Peptides and Proteins under Ultra-High Pressure Conditions
09:20 - Rafael Meinusch: Analysis of Longitudinal and Radial Homogeneity of Monolithic Silica in Capillary Columns by Variation of Capillary Inner Diameter
09:40 - Ken Broeckhoven: Extra-Column Band Broadening Effects in Supercritical Fluid Chromatography: Experimental Measurements and Theoretical Modelling
Session 26: Electromigration
Room 2, Level 0
08:30 -Norman Dovichi: Single Shot Capillary Zone Electrophoresis for Identification of 10,000 Peptides from the HeLa Proteome
09:00 - Charles Lucy: (In)Stability of Adsorbed Cationic Coatings in Capillary Electrophoresis
09:20 -Yi Chen: Direct Capillary Electrophoresis of Dry Samples by Contact Dissolution-Diffusion Injection
09:40 - Norberto Guzman: Coupling Immunoaffinity Capillary Electrophoresis with Sensitivity Detectors for the Characterization of Protein Biomarkers in Biological Fluids and Cells
Session 27: Biopharmaceuticals
Room 3 + 4, Level 0
08:30 -Christian G. Huber: A Top-to-Bottom Approach for In-Depth Characterization of Therapeutic Monoclonal Antibodies
09:00 -Virginie Bettonville: Analysis of Intact Virus-Like Particles of Human Papillomavirus (HPV-VLP) in Capillary Electrophoresis
09:20 -R. Kenneth Marcus: Protein Throughput and Yield Characteristics for Capillary-Channeled Polymer (C-CP) Fibers Phases with Emphasis on the Protein A/IgG System
09:40 - Alain Beck: Cutting-Edge Mass Spectrometry (MS) and LC–MS Methods for Antibody Drug Conjugates Structural Assessment at Multiple Level
Session 28: Fundamentals
Room 1, Level 1
11:00 - Gert Desmet: The Plate Height Concept: Facts and Myths
11:30 - Véronique Wernert: Material Structure Parameters for Transfer Properties
11:50 -Krisztian Horvath: Analysis of Effect of Axial Temperature Gradient on Chromatographic Efficiency
12:10 - Martin Gilar: Impact of Extra-Column Band Broadening on Conventional and Microfluidic LC Performance
Session 29: Electromigration
Room 2, Level 0
11:00 - Frantisek Foret: Practical Considerations for a Hybrid Liquid Junction CE/MS Interface
11:30 -Hailin Wang: Electric Field Distribution in EOF-Driven ITP and t-ITP Revealed by Single DNA Fluorescence Imaging
11:50 - Sam Li: Multiplexed Glycan Analysis by Capillary Electrophoresis
12:10 - Vaclav Kasicka: Affinity Capillary Electrophoresis Applied to Investigation of Noncovalent Interactions of (Bio)molecules
Session 30: Proteins
Room 3 + 4, Level 0
11:00 -Jonathan Bones: Stable Isotope Labeling Strategies for Quantitative Glycomics
11:30 - Trine G. Halvorsen: Dual Immunocapture Combined with Targeted LC–MS–MS for Increased Differentiation Power in Protein Biomarker Determination
11:50 - Therese Wohlschlager: Intact Protein Separation by Ion-Pair Reversed-Phase HPLC: A Platform for the Systematic Characterization of Column Performance
12:10 - Yukui Zhang: Preparation of Ethylene-Bridged Hybrid Silica Monolithic Materials for Protein and Peptide Separation
Presenting GC Data Comparisons to Laypersons to Understand Potential Courtroom Implications
January 6th 2025In forensic science, scientific testimony is routinely presented in court to juries who may have limited or no scientific background, putting law enforcement and defense personnel in the position of having to make rapid decisions in short timeframes based on findings of which they are not specialists. Katelynn Perrault Uptmor, Assistant Professor of Chemistry at William & Mary (Williamsburg, Virginia) believes that the introduction of new technologies into the framework of routine forensic analysis must therefore bridge the gap between introduction of new and novel analytical science and the communication of that science to a court of law, and that analytical chemistry research must be mindful of the need to fill this gap in promoting new technologies.
Detailed Glycosylation Analysis of Therapeutic Enzymes Utilizing Comprehensive 2D-LC–MS
January 3rd 2025In this article, the use of comprehensive two-dimensional liquid chromatography (LC×LC) coupled to mass spectrometry (MS) for characterizing glycosylation of therapeutic enzymes is presented.
Advancing Gene Therapy: Enzyme Selection for Effective RNA Oligonucleotide Mapping
New gene therapy modalities, such as CRISPR guide RNA (single guide ribonucleic acid [sgRNA]) and messenger RNA (mRNA), continue to make progress in both primate and first-in-human trials. As this progress builds, the industry remains accountable for characterizing these molecules to meet the requirements of regulatory authorities.
