Glycan characterization data analysis
Waters has announced that it has entered into an agreement with PREMIER Biosoft International to co-market software produced by the firm with their mass spectrometry solutions for glycan and glycopeptide analysis.
Waters has announced that it has entered into an agreement with PREMIER Biosoft International to co-market software produced by the firm with their mass spectrometry solutions for glycan and glycopeptide analysis. According to the company, by augmenting their separations science products, mass spectrometers and UPLC chemistries with the data analysis software, biopharmaceutical manufacturers can obtain business-critical information critical for understanding biotherapeutic stability and safety.
Glycans are branching polymeric sugar molecules attached to biopharmaceuticals following protein translation and the determination of the sites of glycan attachment and the population of glycans present are required for both therapeutic and safety assessments.
According to the company, its UPLC glycan solution provides high-resolving, reproducible and rapid methods to separate and detect a wide range of glycans. Additionally, when coupled with its mass spectrometers the solution provides additional structural confirmation of the glycans. The software is reported to predict the glycan structure on a protein molecule, score it and generate a list of probable glycans that closely match the given MS profile.
For more information on these products visit the companies’ websites at www.waters.com and www.premierbiosoft.com
The Complexity of Oligonucleotide Separations
January 9th 2025Peter Pellegrinelli, Applications Specialist at Advanced Materials Technology (AMT) explains the complexity of oligonucleotide separations due to the unique chemical properties of these molecules. Issues such as varying length, sequence complexity, and hydrophilic-hydrophobic characteristics make efficient separations difficult. Separation scientists are addressing these challenges by modifying mobile phase compositions, using varying ion-pairing reagents, and exploring alternative separation modes like HILIC and ion-exchange chromatography. Due to these complexities, AMT has introduced the HALO® OLIGO column, which offers high-resolution, fast separations through its innovative Fused-Core® technology and high pH stability. Alongside explaining the new column, Peter looks to the future of these separations and what is next to come.
