This course has been designed for anyone who is either new to LC–MS, or who wishes to expand their current knowledge and understanding about the technique as a whole.
This course has been designed for anyone who is either new to LC–MS, or who wishes to expand their current knowledge and understanding about the technique as a whole.
All aspects of the technology and system are covered - from an overview of a typical HPLC system and important considerations when combining this separation technique with detection by mass spectrometry, all the way through the various atmospheric ionization processes and the different mass analyzers that are available. The main focus is on fundamental concepts but important practical aspects and some basic troubleshooting will also be introduced.
Course Sessions
Session 1: Introduction to LC-MS and API
Session 2: Mass Analyzers
Session 3: HPLC Considerations, Detectors and Vacuum Systems
Session 4: Data Interpretation and Introduction to Method Development
Course Delivery & Duration
· 4 video sessions each session is between 1- 2 hours long
· Tutor supported with access to our chromatography experts
· Supporting training material and downloadable pdf's
· Course assessment with certificate
Our CHROMacademy tutors will deliver four video training sessions over a four-week period with all sessions made available on-demand after their release until the course end date.
It's important you register before the course start date! To gain a certificate of completion you must complete a final assessment before the course end date - this is typically seven weeks after we release session one.
Don't worry if you miss a session you can always catch up at any time within the seven weeks’ period, however we strongly suggest that you keep up to date with the sessions as they are broadcast.
Who should take this course
Anyone who is new to LC–MS, or those wishing to develop their practical understand of LC–MS analysis. This course will provide a full introduction to LC–MS, while also taking knowledge and understanding to a level that will make a real difference in the laboratory!
Fundamentals of Liquid Chromatography-Mass Spectrometry Video Course - September 2015
Amy Claydon BSc PhD - amy@crawfordscientific.com
Reversed Phase HPLC for the Analysis of Biomolecules
November 15th 2016Biopharmaceuticals offer great hope in treating medical conditions which are currently poorly served, at best, by traditional pharmaceuticals. It is estimated that there are over 400 biopharmaceuticals in clinical trials for in excess of 200 disease areas. The enhanced complexity and variability that comes from the size of biopharmaceuticals, allied with the intricacy of the production process, mean chromatography is employed to a much greater extent during production and release testing. The following article will introduce the fundamentals of biopharmaceutical analysis and cover the use of reversed phase HPLC in the analysis of biomolecules. A subsequent article will detail the application of HILIC, IEX, and SEC chromatography for the analysis if biomolecules.