Exploring Ion Mobility–Mass Spectrometry and CIU Data: An ASMS Interview with Brandon Ruotolo
At ASMS 2023, LCGC spoke with Brandon Ruotolo of the University of Michigan in Ann Arbor. This segment is one of several ASMS interviews that were conducted at the conference.
Ruotolo talked with us at ASMS 2023 in Houston, Texas, where he discussed his recently published Analyst paper on ion mobility-mass spectrometry (IM–MS), the reproducibility of collision-induced unfolding (CIU) data, and the impact his research can have on future biomolecular classification efforts.
Ruotolo is the Associate Chair for Research and a Professor of Chemistry at University of Michigan – Ann Arbor, in Ann Arbor, Michigan.
In this interview, Ruotolo answers the following questions:
- Would you explain in basic terms what the focus of your research was in your recent Analyst journal paper on ion mobility-mass spectrometry, and why this research is important?
- Why is ion mobility (IM) resolution currently insufficient for detecting structural differences in large biomolecules?
- How did you investigate the reproducibility of collision-induced unfolding (CIU) data, and what techniques did you use?
- What is the significance of using CIU data when performing native ion mobility-mass spectrometry (IM–MS) research?
- Could you elaborate on the challenges that you faced in this study, particularly when it came to addressing differences in the CIU50 values?
- Based on your findings, what implications do you think this research has for future biomolecular classification efforts?
Our interview with Ruotolo was one of several conducted live at ASMS 2023. Stay tuned for our other interviews conducted at ASMS.
Polysorbate Quantification and Degradation Analysis via LC and Charged Aerosol Detection
April 9th 2025Scientists from ThermoFisher Scientific published a review article in the Journal of Chromatography A that provided an overview of HPLC analysis using charged aerosol detection can help with polysorbate quantification.
Analyzing Vitamin K1 Levels in Vegetables Eaten by Warfarin Patients Using HPLC UV–vis
April 9th 2025Research conducted by the Universitas Padjadjaran (Sumedang, Indonesia) focused on the measurement of vitamin K1 in various vegetables (specifically lettuce, cabbage, napa cabbage, and spinach) that were ingested by patients using warfarin. High performance liquid chromatography (HPLC) equipped with an ultraviolet detector set at 245 nm was used as the analytical technique.
Removing Double-Stranded RNA Impurities Using Chromatography
April 8th 2025Researchers from Agency for Science, Technology and Research in Singapore recently published a review article exploring how chromatography can be used to remove double-stranded RNA impurities during mRNA therapeutics production.
