This application note describes the use of ISOLUTE SLE+ for extraction of estrone (E1) and estradiol (E2) from human serum. Analysis is performed by an HPLC-MS/MS method that gives limits of detection of 0.001 ng/mL without derivatization or fluoride adduct stabilization. ISOLUTE SLE+ products provides high analyte recoveries, no emulsion formation and significantly reduced sample preparation time compared to traditional liquid-liquid extraction.
Pick Your Poison: Isolation of Paclitaxel (Oct 2024)
October 4th 2024The diterpenoid, paclitaxel, which was identified as a potent chemotherapy agent for breast and ovarian cancer originates from the Pacific Yew tree. The isolation of paclitaxel from its major impurities is shown with the use of Hamilton’s PRP-1 (5 µm) HPLC column.