Enantiomeric Separation of Commonly Used Chiral Corn Pesticides Using SFC
Special Issues
The analysis of chiral pesticide is needed to improve risk assessment and establish, in some cases, the justification for production of single- or enriched-enantiomer products. Chiral pesticides are commonly used throughout the agriculture industry and their very chiral nature can lead to enantioselectivities in their mechanisms of action. These differences can also impact their efficacies, toxicities, degradation rates, and persistence in the environment.
This report illustrates good suitability of Regis' chiral columns for analysis of chiral pesticides (Figure 1). While chiral HPLC is a traditional, analytical technique that is performed for this analysis, we report good results using SFC (supercritical fluid chromatography) analysis. SFC is a technique that allows for fast and efficient separations of non-chiral and chiral compounds. It is known for its lower environmental impact, unlike its classical counterpart, liquid chromatography, and therefore could be advantageous.
Experimental Conditions
SFC analysis was performed on the Waters Investigator SFC System. Regis chiral columns - RegisPack, RegisCell, and Whelk-O1 (25 cm × 4.6 mm, 5 µm) - were utilized for method development as the analytical columns.
Conclusion
Chiral separations of commonly used chiral corn pesticides using SFC are reported. Screening was done on chiral columns with various solvent conditions. A set of complementary chiral columns and method development was necessary to achieve selectivity for different compounds. While good results are reported for enantiomeric mixtures, results varied for mixtures of diastereomers and still require further improvement (Figure 2). Compared with traditional HPLC chiral methods, the SFC chiral analyses are usually more efficient. This means methods are faster with good selectivity making SFC the technique of choice.
Regis Technologies, Inc.
8210 Austin Ave., Morton Grove, IL 60053
tel. (847) 583-7661, fax (847) 967-1214
Website: www.registech.com/chromatography
2024 EAS Awardees Showcase Innovative Research in Analytical Science
November 20th 2024Scientists from the Massachusetts Institute of Technology, the University of Washington, and other leading institutions took the stage at the Eastern Analytical Symposium to accept awards and share insights into their research.
Inside the Laboratory: The Richardson Group at the University of South Carolina
November 20th 2024In this edition of “Inside the Laboratory,” Susan Richardson of the University of South Carolina discusses her laboratory’s work with using electron ionization and chemical ionization with gas chromatography–mass spectrometry (GC–MS) to detect DBPs in complex environmental matrices, and how her work advances environmental analysis.
AI and GenAI Applications to Help Optimize Purification and Yield of Antibodies From Plasma
October 31st 2024Deriving antibodies from plasma products involves several steps, typically starting from the collection of plasma and ending with the purification of the desired antibodies. These are: plasma collection; plasma pooling; fractionation; antibody purification; concentration and formulation; quality control; and packaging and storage. This process results in a purified antibody product that can be used for therapeutic purposes, diagnostic tests, or research. Each step is critical to ensure the safety, efficacy, and quality of the final product. Applications of AI/GenAI in many of these steps can significantly help in the optimization of purification and yield of the desired antibodies. Some specific use-cases are: selecting and optimizing plasma units for optimized plasma pooling; GenAI solution for enterprise search on internal knowledge portal; analysing and optimizing production batch profitability, inventory, yields; monitoring production batch key performance indicators for outlier identification; monitoring production equipment to predict maintenance events; and reducing quality control laboratory testing turnaround time.
Infographic: Be confidently audit ready, at any time and reduce failures in pharma QC testing
November 20th 2024Discover how you can simplify the audit preparation process with data integrity dashboards that provide transparency to key actions, and seamlessly track long-term trends and patterns, helping to prevent system suitability failures before they occur with waters_connect Data Intelligence software.
Critical Role of Oligonucleotides in Drug Development Highlighted at EAS Session
November 19th 2024A Monday session at the Eastern Analytical Symposium, sponsored by the Chinese American Chromatography Association, explored key challenges and solutions for achieving more sensitive oligonucleotide analysis.
