Scientists from the Chinese Academy of Agricultural Sciences in Beijing, China developed a new method for detecting 20 antihistamines in raw cow’s milk samples using high-field quadrupole Orbitrap high resolution mass spectrometry (UHPLC-QE HF HRMS) combined with dispersive micro solid phase extraction (DMSPE). Their findings were published in the Journal of Chromatography A (1).
Can container milk with cow milking facility and mechanized milking equipment in the milking hall | Image Credit: © thanapun - stock.adobe.com
This method was applied to 205 collected cow’s milk samples, with eight antihistamines being detected in 16 milk samples. There were 28.2 μg/L~157.9 μg/L of chlorphenamine, 254 μg/L of cyproheptadine, and of 25.7 μg/L promethazine in the samples.
Antihistamines are medicines that are typically used to relieve allergy symptoms, such as hives, hay fever, and reactions to insect bites (2). They are antagonists of histamine receptors and are divided into three main groups, otherwise known as generations: first-generation, which can pass into the brain more easily and are more likely to cause drowsiness, second-generation, which are newer and less likely to cause drowsiness, and third-generation, which are made from second-generation antihistamines and are even less likely to cause drowsiness (3).
Due to the antiemetic, sedative, and hypnotic effects of antihistamines, these medications have been illegally used for animal breeding to promote growth and treat inflammation and allergies. Additionally, antihistamines can be used as veterinary drugs to reduce the pressure and mortality during animal transportation, treat diseases, and improve the efficacy of antibacterials.
However, antihistamines have strong central nervous system inhibitory effects, with some second-generation antihistamines having been found to have significant cardiac toxicity. Moreover, antihistamines can be excreted into milk during breastfeeding. Exposure to antihistamines during breastfeeding can lead to irritability, colic, sleepiness, and in extreme cases can lead to death. There have only been a handful of published studies focused on the levels of antihistamine residue in milk and the evaluation of risks due to deficiency of trace analysis methods, according to the scientists (1).
Various methods have been developed for tracking antihistamines in water, blood, urine, and drug formulations; however, there is very little literature describing the determination of antihistamines in milk, using techniques like gas chromatography-flame ionization detector (GC-FID), radioimmunoassay (RIA), and GC–mass spectrometry (GC–MS). Using these with a traditional detector can make it difficult to detect trace antihistamines due to the presence of additional interference peaks and low concentrations of analytes from the milk matrix.
In the Journal of Chromatography A study,the scientists determined the limit of detection (LOD) for 20 antihistamines in milk ranged from 0.05 µg/L to 1.0 µg/L. Recoveries were between 80.7% and 108.3%, with the relative standard deviation being less than 15%. The scientists found 1.3 μg~8.5 μg/L of chlorphenamine, diphenhydramin, desloratadine, cetirizine, loratadine, and ebastine were found in 11 milk samples. These residuals could have stemmed from antihistamine use in animal husbandry, pollution during breeding, antihistamine residues in feed and drinking water, or the breeding environment.
Altogether, this proposed method proved suitable for confirmatory monitoring and quantitative analysis of antihistamines in milk. This may be noteworthy for protecting human health and fighting environmental pollution. However, further research is needed, as antihistamines in cow’s milk has not been extensively studied. Existing detection methods are not yet being specific or sensitive enough to accurately detect these residues, the scientists wrote. Further studies could help develop a better understanding of the impact of antihistamine residues on human health.
(1) Decheng, S.; Zhanteng, S.; Zhiming, X.; et al. Trace Analysis of 20 Antihistamines in Milk by Ultrahigh Performance Liquid Chromatography Coupled with High Field Quadrupole Orbitrap High Resolution Mass Spectrometry Followed Dispersive Micro Solid Phase Extraction. J. Chromatogr. A 2024, 1727, 464989. DOI: 10.1016/j.chroma.2024.464989
(2) Antihistamines. Crown 2024. https://www.nhs.uk/conditions/antihistamines/ (accessed 2024-6-17)
(3) Foley, K. Benadryl, Zyrtec, and More: A List of 7 Antihistamines. GoodRx, Inc 2024. https://www.goodrx.com/classes/antihistamines/drugs-list (accessed 2024-6-17)
Study Examines Impact of Zwitterionic Liquid Structures on Volatile Carboxylic Acid Separation in GC
March 28th 2025Iowa State University researchers evaluated imidazolium-based ZILs with sulfonate and triflimide anions to understand the influence of ZILs’ chemical structures on polar analyte separation.
Analytical Challenges in Measuring Migration from Food Contact Materials
November 2nd 2015Food contact materials contain low molecular weight additives and processing aids which can migrate into foods leading to trace levels of contamination. Food safety is ensured through regulations, comprising compositional controls and migration limits, which present a significant analytical challenge to the food industry to ensure compliance and demonstrate due diligence. Of the various analytical approaches, LC-MS/MS has proved to be an essential tool in monitoring migration of target compounds into foods, and more sophisticated approaches such as LC-high resolution MS (Orbitrap) are being increasingly used for untargeted analysis to monitor non-intentionally added substances. This podcast will provide an overview to this area, illustrated with various applications showing current approaches being employed.
Advances in Non-Targeted Analysis for PFAS in Environmental Matrices
March 27th 2025David Megson from Manchester Metropolitan University in Manchester, UK, spoke to LCGC International about the latest developments in non-targeted analysis (NTA) of per- and polyfluoroalkyl substances (PFAS) in environmental matrices based on a recent systematic review paper he has collaboratively published (1).
Study Explores Thin-Film Extraction of Biogenic Amines via HPLC-MS/MS
March 27th 2025Scientists from Tabriz University and the University of Tabriz explored cellulose acetate-UiO-66-COOH as an affordable coating sorbent for thin film extraction of biogenic amines from cheese and alcohol-free beverages using HPLC-MS/MS.
Quantifying Microplastics in Meconium Samples Using Pyrolysis–GC-MS
March 26th 2025Using pyrolysis-gas chromatography and mass spectrometry, scientists from Fudan University and the Putuo District Center for Disease Control and Prevention detected and quantified microplastics in newborn stool samples.