Best of the Week: HPLC Recap, Ion Chromatography, Organ-on-a-chip Systems, and More

This week, LCGC International published a variety of articles on the hottest topics in chromatography and beyond. Below, we’ve highlighted some of the most popular articles, according to our readers. Happy reading!

Analytically Speaking, Ep. 26: HPLC 2024 Conference Recap

Dwight Stoll, Jim Grinias

In this episode of “Analytically Speaking,” the podcast from LCGC International and Spectroscopy, podcast co-hosts Dwight Stoll and James Grinias discuss highlights and observations from the recently completed HPLC 2024 that was held in Denver, Colorado in July. The “HPLC Meeting”, as the conference is affectionately known by regular attendees, is widely regarded as the premier venue to discuss the latest advances in liquid chromatography research and applications, and is well attended by influencers in the community from both academic and industrial research laboratories. This year’s meeting was no exception, with fantastic talks and posters presented by scientists from around the world. In the conversation, they discuss some of the major themes and trends we observed at the meeting, including the proliferation of research around therapeutic oligonucleotides, and the increasing emphasis on the “green-ness” of analytical science in general, and liquid phase separations in particular.

Advancing Pharmaceutical Analysis with Ion Chromatography

Elke Süss

Ion chromatography (IC) is becoming increasingly important for pharmaceutical applications. In contrast to other technologies, IC can resolve multiple ionic species and polar analytes simultaneously. Coupling to other methods, such as mass spectrometry, offers even more sensitivity. The high degree of automation possibilities makes IC ideal for routine quality control of drugs in bulk and pharmaceutical dosage forms. In many cases, IC has been qualified for US Pharmacopeia standards, making it a vital tool in this field. Since 1975, the primary goal of the United States Pharmacopeia-National Formulary (USP–NF) has been to establish minimum testing requirements throughout pharmaceutical development and manufacturing processes to ensure drug efficacy and patient safety, and they have introduced multiple standardized procedures from this work. The modernization initiative from the USP and U.S. Food and Drug Administration (FDA), which began in 2010, has so far resulted in approximately 4300 monographs and 220 general chapters updated with state-of-the-art analytical technology (5). LC techniques are broadly applied to pharmaceutical and biopharmaceutical products (1,6) and are now cited in these monographs and chapters.

Rapid Quantitative Analysis of Ethylene Oxide and 1,4-Dioxane in Polymeric Excipients Using SIFT-MS

Juby Mathew, Vaughan S. Langford, Chad Bastian, Christopher Williams, Alyssa McBurney, Mark J. Perkins

Pharmaceutical excipients, such as polyethylene glycol-based polymers, must be tested for the presence of ethylene oxide (EtO) and 1,4-dioxane as part of a safety assessment, according to USP Chapter <228>. Selected ion flow tube mass spectrometry (SIFT-MS) offers advantages over conventional GC methods and accelerates quantitative and selective analysis of EtO and 1,4-dioxane through elimination of a lengthy matrix-match preparation and shorter run times providing 9- to 14-fold higher daily throughput than gas chromatography (GC). These efficiencies are gained while maintaining high analytical sensitivity, using 10-fold less sample per analysis.

Self-Cleaning LC–MS in Organoid/Organ-on-Chip Systems for Small Molecule Analysis

Alasdair Matheson

In this interview, Steven Ray Wilson and Hanne Røberg-Larsen, both from the University of Oslo in Norway, discuss the practical application and advantages of their FDA-validated research to analyse small molecule drugs and their metabolites in organoid/organ-on-chip systems that serve as alternatives to animal models.

The 28th International Symposium on Separation Sciences (ISSS 2024)

Alasdair Matheson

The 28th International Symposium on Separation Sciences (ISSS 2024), chaired by Luigi Mondello (University of Messina, Italy) and Danilo Corradini (CNR-Institute for Biological Systems, Rome, Italy), will be held in Messina on September 22–25, 2024. In this interview, Corradini, who is a member of the permanent Scientific Committee of ISSS, reveals what the conference will offer participants this year.