Analytical Method Development for Reversed-Phase HPLC

The diterpenoid, paclitaxel, which was identified as a potent chemotherapy agent for breast and ovarian cancer originates from the Pacific Yew tree. The isolation of paclitaxel from its major impurities is shown with the use of Hamilton’s PRP-1 (5 µm) HPLC column.

Garlic has been found to exhibit a multitude of proliferative health benefits in the realm of cardiac events.

A reversed-phase HPLC method was developed to separate five steroid hormones, with partition coefficients ranging from 1.47 (cortisone) to 4.5 (pregnenolone).

Sulfide compounds found in garlic oils are used in therapeutics and prophylaxis of certain cancers, and are prevalent in cell health.

Identification of the five most common saccharides found in packaged foods, lactose, maltose, sucrose, fructose, and glucose, is easily resolved on the RCX-30 column.

The Raffinose Family of Oligosaccharides (RFO) is comprised of low molecular weight non-reducing sugars. Analysis of these compounds is not easily done unless using HC-75.