All HPLC C18s Are the Same Aren't They?
It's just a silica support with a C18 bonded on, surely there can't be much difference between them? Wrong.
It’s just a silica support with a C18 bonded on – surely there can’t be much difference between them? Wrong. There are huge differences between standard C18 phases and simply swapping one C18 for another, without accounting for these differences, will produce differences in retention time, resolution and peak elution order. Most of us are aware of the difference between endcapped / non-endcapped columns or the idea of carbon loading. Although this is a start, differences in selectivity are caused by many complex factors and none of the manufacturers have established a standardized system of quantifying the effects of these differences.
The following is an overlay of 4 modern standard C18 columns by popular manufacturers:
The complete article is available to CHROMacademy Lite and Premier members here >>
CHROMacademy Lite Membership is FREE and it only takes two minutes to register.
With a Lite Membership you are given access to:
∙ This month's webcast & tutorial
∙ Selected eLearning modules
∙ Featured CHROMacademy Content
∙ The CHROMacademy forum
Test drive CHROMacademy and Check out more great content available FREE to our Lite members »
Best of the Week: Food Analysis, Chemical Migration in Plastic Bottles, STEM Researcher of the Year
December 20th 2024Top articles published this week include the launch of our “From Lab to Table” content series, a Q&A interview about using liquid chromatography–high-resolution mass spectrometry (LC–HRMS) to assess chemical hazards in plastic bottles, and a piece recognizing Brett Paull for being named Tasmanian STEM Researcher of the Year.
Using LC-MS/MS to Measure Testosterone in Dried Blood Spots
December 19th 2024Testosterone measurements are typically performed using serum or plasma, but this presents several logistical challenges, especially for sample collection, storage, and transport. In a recently published article, Yehudah Gruenstein of the University of Miami explored key insights gained from dried blood spot assay validation for testosterone measurement.
Determination of Pharmaceuticals by Capillary HPLC-MS/MS (Dec 2024)
December 19th 2024This application note demonstrates the use of a compact portable capillary liquid chromatograph, the Axcend Focus LC, coupled to an Agilent Ultivo triple quadrupole mass spectrometer for quantitative analysis of pharmaceutical drugs in model aqueous samples.
