Advanced Proteometic Workflows Using micro Pillar Array Columns: Insights of “The Proteome Landscape of the Kingdom of Life

Register free: https://www.chromatographyonline.com/lcgc_w/proteome_landscape​

Event Overview:

Reverse-phase liquid chromatography is the most commonly used separation method for shotgun proteomics. Nanoflow-chromatography has emerged as the preferred chromatography method for increased sensitivity and separation, allowing the detection of a higher number of IDs with reduced needs for sample material.

Micro-chip based pillar array chromatography columns are a novel form of nanoflow-separation columns, which bring significant benefits for the researcher. In contrast to conventional LC columns that contain randomly packed beads as their stationary phase, micro-chip -based pillar array chromatography columns have a separation bed of perfectly ordered and free-standing pillars obtained by lithographic etching of a silicon wafer. The regular mobile phase flow pattern through these micro-chip pillar array columns adds very little dispersion to the overall separation, resulting in better peak resolution, sharper elution peaks and increased sensitivity. The free-standing nature of the pillars also leads to much lower back pressure buildup, and makes it possible to operate longer columns at moderate system pressures.

Researchers from the Mann lab (Max Planck Institute, Munich) used an advanced proteomics workflow for the in-depth study of 100 taxonomically diverse organisms. With two million peptide and 340,000 stringent protein identifications obtained in a standardized manner, they were able to double the number of proteins with solid experimental evidence known to the scientific community. The data also provide a large-scale case study for sequence-based machine learning, which has been demonstrated by experimentally confirming the predicted properties of peptides from Bacteroides uniformis.

In this webcast, Dr. Geert Van Raemdonck of PharmaFluidics will explain the principles of the micro-chip based pillar array columns and demonstrate how the PharmaFluidics µPAC™ columns can contribute to develop advanced proteomic workflows. Drs. Johannes Mueller from the Max Planck Institute in Munich will explain how this Nature publication has been concluded and how the 200 cm long micro Pillar Array Columns have contributed to establish this large dataset.

Key Learning Objectives:

To learn the principles of micro-chip-based pillar array chromatography columns.

• To learn the principles of micro-chip-based pillar array chromatography columns.
• To understand the benefits and separation performance of micro-chip-based chromatography columns for the analysis of various biological samples.
• To demonstrate real-world applications of the retention time stability and reproducibility of the micro-chip pillar array chromatography.
• To learn the importance of having large proteome datasets available

Speakers: Dr. Geert Van Raemdonck, Global Field Support Manager, PharmaFluidics

Drs. Johannes Mueller, PhD Student, Mann lab (MPI of Biochemistry)

Time and Date: Tuesday, October 13, 2020 at 9am EDT| 2pm BST| 3pm CEST

On demand available after final airing until Oct. 13, 2021

Sponsor: PharmFluidics

Register free: https://www.chromatographyonline.com/lcgc_w/proteome_landscape​

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