The Column-06-06-2017
Intelligent Peak Deconvolution
June 6th 2017High quality, low cost is a standard mantra within the pharmaceutical industry, but with increasing structural complexity of drugs and drug candidates maintaining the core value of this mantra is becoming more difficult. Kanta Horie from Eisai Co., Ltd., recently spoke to The Column about the development of an intelligent peak deconvolution technique using multivariate curve resolution-alternating least squares (MCR-ALS) that allows accurate quantitation of multiple components with different absorbing spectra even if the peaks are not completely separated.
Novel Sampling Technique For Sex Pheromone Analysis in Air
June 6th 2017A novel unit that integrates sampling and analysis for the determination of pest insect sexual pheromones in environmental air using fabric phase sorptive extraction (FPSE) and headspace gas chromatography coupled to mass spectrometry (HSGC–MS) has been developed at the University of Córdoba in Spain.
Tips & Tricks GPC/SEC: Product Registration and REACH
June 6th 2017Molecular mass is one of the central parameters required for product registration. Compared to low molar mass substances, the molar mass determination of macromolecular products is more difficult because the product is a mixture of chains with different lengths and, therefore, molar masses. Gel permeation chromatography/size-exclusion chromatography (GPC/SEC) is the standard technique to separate macromolecules by size and to measure the complete molar mass distribution as well as the molar mass averages. This technique therefore provides crucial information for product registration, including REACH.
Analysis of Residual Antimicrobials in Meat Using HPLC
June 6th 2017Analysis of residual antimicrobials in animal products is crucially important to ensure food safety. This article presents a simple, fast, and highly sensitive high performance liquid chromatography (HPLC) assay of regulated antimicrobials, as well as sample preparation and purification methods. Twenty-four different analytes of interest were investigated in beef, pork, and chicken meat samples.
Enantiomeric Separation of Privileged Scaffold Derivatives in Early Drug Discovery Using Chiral SFC
June 6th 2017Successful therapeutic intervention often requires chiral medicines because of the intrinsic chirality of protein drug targets, which consist of L-amino acids. Potency, efficacy, and safety can be highly dependent on the precise stereochemical geometry of the molecules. Determining the biological profile of individual enantiomers in the early stages of drug discovery is important for successful optimization towards clinical candidates. Here we demonstrate the benefits of supercritical fluid chromatography (SFC) with three chiral stationary phases exemplified by high frequency resolution of 41 out of 50 chiral derivatives of eight commonly used drug discovery scaffolds including 1,3-thiazoles, 1,3-benzothiazoles, pyranoquinolones, indoles, and leucolines.
