Application Notes: Biological, Medical, and Clinical
Analysis of Fentanyl and Its Analogues in Human Urine by LC–MS/MS
December 1st 2018Abuse of synthetic opioid prescription painkillers such as fentanyl, along with a rapidly growing list of illicit analogues, is a significant public health problem. In this study, we developed a simple dilute-and-shoot method that provides a fast 3.5-min analysis of fentanyl and related compounds (norfentanyl, acetyl fentanyl, alfentanil, butyryl fentanyl, carfentanil, remifentanil, and sufentanil) in human urine by LC–MS/MS using a Raptor Biphenyl column.
Analysis of Phospholipid Classes by iHILIC®-Fusion
September 1st 2018Phospholipids (PLs) are the major components of cellular membranes. They are important for the functionality of membrane proteins or serve as precursors for second messengers. Several studies reveal the role of PL alterations in various diseases such as cancer (1). Therefore, it is crucial to identify and quantify PLs in complex biological samples for lipidomic studies and clinical research.
Analysis of Fentanyl and Its Analogues in Human Urine by LC–MS/MS
September 1st 2018Abuse of synthetic opioid prescription painkillers such as fentanyl, along with a rapidly growing list of illicit analogues, is a significant public health problem. In this study, we developed a simple dilute-and-shoot method that provides a fast 3.5-min analysis of fentanyl and related compounds (norfentanyl, acetyl fentanyl, alfentanil, butyryl fentanyl, carfentanil, remifentanil, and sufentanil) in human urine by LC–MS/MS using a Raptor Biphenyl column.
Monitoring Emissions From Respiratory Medical Devices in Accordance With ISO 18562-3
December 1st 2017This study describes the monitoring of potentially harmful volatile organic compounds (VOCs) emitted from respiratory medical devices, by pumped sampling and thermal desorption–gas chromatography–mass spectrometry (TD–GC–MS) analysis in accordance with ISO 18562 part 3. Emissions from two sets of face-mask supply tubing and three nasal cannulas were compared, and all were found to emit VOCs at levels that may give cause for concern.
Definitive EtG/EtS LC–MS/MS Analysis: A Rugged 4-Min Method for High‑Throughput Laboratories
December 1st 2017Methods for monitoring alcohol consumption biomarkers EtG and EtS are generally limited by poor retention and coelution with matrix interferences, as well as by long analysis times and short column lifetimes. The dilute-and-shoot EtG/EtS LC–MS/MS analysis developed here using the novel Raptor EtG/EtS column easily resolves EtG and EtS from matrix interferences, providing consistent, accurate results for high-throughput labs testing human urine samples for alcohol consumption.
Advantage of Premixed Mobile Phases for Consistent Chromatography of Peptides Using Nano-LC–MS/MS
May 1st 2017High resolution mass spectrometry with nano-LC is used for protein identification and quantification in both top-down and bottom-up proteome analysis. Reliable instrumentation in combination with ultrapure mobile phases is essential for data integrity. Premixed 80% acetonitrile with 0.1% formic acid (LS122-500) and water with 0.1% formic acid (LS118-500) were designed to produce a consistent chromatographic performance using this instrument system. In this study, these mobile phases were used extensively to evaluate several factors which can affect separation of protein digests such as peak retention, peak repeatability, and sample carryover. Our results demonstrated excellent chromatographic performance using Thermo Scientific EASY-nLC 1200 LC system and Thermo Scientific LTQLX ™ ion trap mass spectrometer with the specialized premixed mobile phases.