Market Profile: UHPLC
Ultrahigh-performance liquid chromatography (UHPLC) systems are designed to take full advantage of smaller particles (sub 2 μm), which yield higher resolution and increased sensitivity. Because of increased back pressure, UHPLC systems employ high-performance pumps that deliver 15 000 psi (1,000 bar) or more of pressure. Some systems solve the back-pressure issue by elevating temperatures using a column oven, which lower the viscosity of the mobile phase, allowing it to move faster through the column. UHPLC systems have been shown to provide analysis times of less than 1 min and up to 40 times faster than conventional HPLC systems.
PLS-DA With Univariate Filtration Refines Discovery of Untargeted Metabolomics Data Using LC–MS
May 23rd 2024As opposed to targeted metabolomics which looks only at a predetermined set of metabolites or their pathways, an untargeted approach was preferred by this research team because of its unbiased yet still comprehensive nature.