May 31st 2024
In preparation for ASMS 2024, we recently interviewed Uri Keshet of the University of California, Davis about his upcoming presentation and expectations for the conference.
Quantitative Analysis of 25-OH-D2 and 25-OH-D3 in Serum by LC-MS-MS
March 30th 2008Vitamin D is a group of fat-soluble prohormones that helps the body absorb calcium and maintain proper levels of calcium and phosphorus in the blood. A vitamin D deficiency results in impaired bone mineralization and leads to bone-softening diseases, such as rickets in children and osteomalacia and osteoporosis in adults, as well as autoimmune and nervous system diseases. A vitamin D overdose can raise blood levels of calcium and cause gastrointestinal symptoms and kidney disease. High blood levels of calcium also can cause heart rhythm abnormalities and calcinosis, the deposition of calcium and phosphate in the body's soft tissues.
Atmospheric Pressure Photoionization — The Second Source for LC-MS?
March 1st 2008This article explores the progress that atmospheric pressure photoionization (APPI) has made in its relatively short history for LC–MS analysis. Specifically, the authors examine the combination of APPI and electrospray ionization (ESI).
Successful Extraction of Basic Drugs from Plasma Using Mixed-Mode Solid-Phase Extraction
February 29th 2008This article aims to demonstrate the unique capability of resin-based mixed-mode solid-phase extraction (SPE) to achieve efficient extraction of basic drugs from plasma. Optimized pore size of the method minimizes retention of high molecular weight matrix components present in the biological fluid samples. The interferences are removed, ensuring extremely clean extracts for subsequent liquid chromatography?mass spectrometry-mass spectrometry (LC-MS-MS) analysis.
In Vino Veritas: LC–MS in Wine Analysis
December 1st 2007Liquid chromatography–mass spectrometry (LC–MS) is a popular technique for the analysis of wine. This article gives an overview of wine analysis and new insights this technique has revealed regarding the composition of wine, possible health benefits, customer safety and the understanding of winemaking processes.
Why Everyone Should Have Silica Hydride Based HPLC Columns in Their Lab
October 30th 2007The unique features of silica hydride-based columns are described with a focus on how these columns can expand a laboratory's capabilites in HPLC and LC-MS. Of particular interest is the ability to retain both polar and nonpolar compounds and provide an orthogonal method of analysis for currently existing procedures.
Fast LC-MS-MS Quantitation of N-Methyl Carbamate Pesticides in Food
August 30th 2007A fast quantitation method for the analysis of N-Methyl Carbamate pesticide residues in vegetables and grains has been developed. High recoveries were obtained for most carbamates screened in all matrices tested at 1 ppb levels. Good linearity of the calibration curves was observed for all analytes, over the range from 1 ppb to 200 ppb levels, with r2 greater than 0.99.
High-Throughput Quantitative LC-MS-MS Assays by On-Line Extraction Using Monolithic Support
April 1st 2007LC-MS-MS has become a widely used technique for the fast and sensitive quantitation of small molecules. In this article, this approach has been extended to high-throughput quantitative LC-MS-MS analysis under GLP applications for a drug candidate in development from preclinical animal studies through clinical development.
New Sample Fractionation Strategies for Proteomic Analyses by LC–MS
November 1st 2006Mass spectrometry has long been a preferred tool for protein identification and biomarker discovery, but preparation of biological samples remains a challenge. Hindrances include the wide range of protein concentrations, sample complexity, and loss or alteration of important proteins due to sample handling. This article describes recent developments in sample fractionation technologies that are overcoming these challenges in interesting ways and are enabling in-depth proteomic studies that were not possible in the past.
High-Stakes Competition: Advances in Chromatography Stay Ahead of Dopers
September 29th 2006Doping testing is now an accepted fact of sporting life - whether in major international competitions, such as the Olympics, or in regular events at the national level. Athletes who cheat by taking banned substances risk harming their careers and their health. They also bring their country and their sport into disrepute. A reliable and reproducible scientific system for doping testing, backed up by sanctions, helps fight the culture of drugs in sport so that athletes can participate on a level playing field.
Top-Down versus Bottom-Up Approaches in Proteomics
September 1st 2006September 2006. Top-down and bottom-up are alternative strategies for protein identification and characterization by mass spectrometry. How do they fit into the world of proteomics? What are their implications for separation technology? These questions are addressed in this installment of "Directions in Discovery."
Glossaries, Dictionaries, and Understanding Mass Spectrometry
February 1st 2006Definitions, however meticulously crafted, can give the meaning of terms with scrupulous clarity, but often fail to tell us how to apply them. Such definitions are descriptions that dictate usage but stop well short of the sense and purpose that many of us turn to them for.
Evaluating Analytical Instrumentation Using the Golf Score Card Tool
September 1st 2005The use of a simple Excel-based "Golf Score Card" tool can facilitate assessments and instrument acquisistion decisions by collating and weighting the relative importance of the many criteria that impact the final purchase decision. The authors describe this process.