In this first roundtable discussion focused on Women in Chromatography, LCGC International spoke with Diane Turner, Jacqueline Hamilton, and Caroline Gauchotte-Lindsay, who are all leading voices in the LinkedIn International Women in Chromatography group.

In this second part of our interview with Females in Mass Spectrometry (FeMS) Empowerment Award Winner Josephine Ouma, we discuss her experience with mass spectrometry and how FeMS has impacted her life.

A study conducted at Henan University of Science and Technology (Luoyang, China).aimed to characterize the pharmacokinetic profiles of milbemycin oxime in Pekingese dogs following a single oral (PO) and intravenous (IV) dose. Blood samples were collected at various time points, with milbemycin oxime concentrations measured using a validated high performance liquid chromatography (HPLC) method with ultraviolet (UV) detection.

Emanuela Gionfriddo and Madison Williams from University at Buffalo, the State University of New York, NY, USA discuss the important role that solid-phase microextraction (SPME) techniques with gas chromatography mass spectrometry (GC–MS) can play in the analysis of per- and polyfluoroalkyl substances (PFAS).

In this first part of our interview with Females in Mass Spectrometry (FeMS) Empowerment Award Winner Josephine Ouma, we discuss her PhD research and what inspired her to join FeMS.

Research on plastics in aquatic environments is well-established, but little is known about their presence, pathways, and effects in soil ecosystems. The iMulch project aims to address this need as well as the growing concern of plastic pollution in soils. Focusing on developing and validating methods to detect micro- and macroplastics in soils and drainage waters, with an emphasis on how drainage water impacts municipal wastewater treatment, a key aspect of the study is analyzing plastic emissions from agricultural mulch films. We spoke to Carmen Wolf of the Institute for Environment and Energy, Technology and Analytics e.V. (IUTA) in Duisburg, Germany, corresponding author of a recent paper on the project, about the investigations involved and how separation science was used in their experimentation.

The researchers explored ways to simplify retention time calculation across LC–MS and various other chromatographic methods.

A new type of micro-gas chromatography column was fabricated using direct metal laser sintering (DMLS) to circumvent manufacturing issues faced by other micro-GC systems.

From June 12–13, 2025, the 3rd Advances in Separation Science Workshop will take take place in Gembloux, Belgium.

Here's a taster of what to look forward to at the 54th International Symposium on High Performance Liquid Phase Separations and Related Techniques (HPLC 2025), which will be held from Sunday through Thursday, 15–19 June, 2025, in Bruges, Belgium.

LCGC International spoke with Daniel Meston and Dwight Stoll from Gustavus Adolphus College in St. Peter, Minnesota, USA, about a project they worked on with Todd Maloney from Eli Lilly in Indianapolis, Indiana, USA, to investigate the optimal performance conditions of antisense oligonucleotides (ASOs) when using hydrophilic interaction liquid chromatography (HILIC).

Sample concentration is a parameter that can influence the quality of gel permeation chromatography/size-exclusion chromatography (GPC/SEC) separations and the obtained results. Understanding this influence can help to support the development of reliable GPC/SEC methods.

This article introduces a multi-step preparative purification workflow for synthetic peptides using liquid chromatography–mass spectrometry (LC–MS). The process involves optimizing separation conditions, scaling-up, fractionating, and confirming purity and recovery, using a single LC–MS system. High purity and recovery rates for synthetic peptides such as parathormone (PTH) are achieved. The method allows efficient purification and accurate confirmation of peptide synthesis and is suitable for handling complex preparative purification tasks.

This article discusses several practical examples using bioinert columns, demonstrating their ability to deliver reliable, high-resolution results with precision and reproducibility.

How is the indoor air affecting your health?