Application Notes: General

Protein standards explored with known hydrodynamic radii, show how this can determine ideal size-exclusion conditions for optimal method performance using Biozen columns.

This validation study demonstrates the use of the QSight® tandem quadrupole mass spectrometer (LC–MS/MS) for the application of PFAS EPA Method 537.1 in drinking water.

This application note explores the effect of column internal diameter (4.8 and 7.6 mm IDs) for robust size-exclusion chromatography methods.

In this application note, an HPLC with MWD detection method is detailed to detect parabens in cosmetic and personal care products.

Exploring the use of a standard phosphate containing buffer for aggregate analysis of an IgG2 mAb, Panitumumab, which has known recovery issues due to its hydrophobicity

Size-exclusion method improvements by running at a lower flow rate for the aggregate analysis of NIST mAb using a Biozen dSEC-2 column

The robustness of a sub-2 µm Biozen dSEC-2 column is demonstrated by displaying over 300 injections of a protein sample (Myoglobin).

This application, using a Biozen dSEC-2 column, demonstrates the effect of organic solvent of an ADC “mimic" on the analysis.

A simple, fast, robust green chemistry method for the accurate quantitation of six additives in diet soft drinks using HPLC with photodiode array (PDA) detection.

NIST mAb was analyzed using six different batches of 1.8 µm 200 Å dSEC-2 media packed with 150 mm length and 4.6 mm inner diameter columns.

This application note highlights the use of solid-phase extraction (SPE) coupled with LC–MS/MS for sample enrichment and quantitation of endocrine-disrupting chemicals.

This application note describes a rapid LC–MS/MS method for the separation and detection of 44 drug of abuse compounds in urine samples.

SEC–HRMS demonstrated for the analysis of an IdeZ digested mAb for specific identification of any posttranslational modifications specific to the hypervariable region.

This application note describes two methods for isopropyl alcohol impurities detection: USP recommended 42-min analysis, and a speed-optimized method in less than 6 min.

UniSpray is a novel atmospheric ionization technique that provides increased peak response, enabling ultra-trace detection of PFAS and other contaminants.

Purification of peptide-peptoid hybrids can be obtained by reversed-phase chromatography with excellent recovery and resolution.

The ability to collect compounds is related to the ability to detect them. Many possess weak or non-existent chromophores, causing UV detection to exhibit poor yields. 

Purification of a single-stranded RNA oligonucleotide by ion pair reversed-phase HPLC is demonstrated, achieving > 99% pure fraction pool with a yield of > 56%.

This app note introduces the use of the Nexera XS inert UHPLC, which utilizes a metal-free flow path for accurate and reproducible analysis of nucleotides.  

This technical report covers how a column packed with small particle material can be used to optimize an analytical method for monoclonal antibody aggregates.